用EROD研究苯并芘和六氯苯的联合毒性作用

Application of EROD in Studying Benzo(a)pyrene and Chlorobenzene Combined Toxicity

用7-乙氧基异叻唑酮-脱乙基酶(EROD)检测的方法,研究了苯并芘和六氯苯对日本青鳉肝脏EROD酶的比活力的影响。结果表明,苯并芘和六氯苯对EROD酶的比活力均有激活作用,在实验浓度范围内,EROD酶的比活力与两者浓度之间存在剂量-效应关系。苯并芘和六氯苯表现为一定的协同作用。实验同时发现日本青鳉在六氯苯和苯并芘中暴露后,EROD酶的比活力开始有一个短暂的降低,然后持续升高。对六氯苯和苯并芘暴露的最佳时间进行了探讨。

Induction of EROD activity was investigated by an in vitro assay based on benzo(a)pyrene[B (a)P] and hexa chlorobenzene . Both of them were found to induce the activity of EROD. There was a dose-effect relationship between EROD activity and concentrations of the two chemicals. A combined toxicity of benzo(a)pyrene and hexa chlorobenzene was observed. When Japanese medaka was exposed to the two chemicals, EROD activity kept rising after a brief fall. In this article, the combined toxicity and optimal exposed time of benzo(a)pyrene and hexa chlorobenzene were also studied by EROD analysis.