木瓜蛋白酶酶解鱼鳞提取胶原蛋白的工艺研究

Research on Extracting Technology of Collagen Protein Cells from Fish Scale with Enzyme of Papainase

用木瓜蛋白酶水解鱼鳞提取胶原蛋白,对影响酶解过程的的主要因素(酶量、温度、底物浓度)分别作为单因素进行了实验。并通过正交实验得到鱼鳞胶原蛋白提取条件的优化组合,同时对提取物进行了分析检测。结果表明,木瓜蛋白酶对鱼鳞有较好的水解效果,酶用量宜采用4g/L,最佳温度为60℃,底物浓度宜选择20%。

The hydrolysis conditions of fish scale for extracting papainase have been studied.An experi- ment has been performed to determine the main factors that affect the zymohydrolysis process,in which enzyme amount,temperature and substrate concentration,as individual factors,have been tested.An orthogonal test also has been carried out to obtain the optimum conditions that the enzyme dosage is 4 g/L,the hydrolyzing temperature is 60℃and the substrate concentration is 20%.