丹参对顺铂耳蜗毒性的防护作用

The Protective Effect of Salvia Miltiorrhiza on Osplatin Ototoxicity

目的探讨丹参对顺铂中毒豚鼠耳蜗诱导性一氧化氮合酶表达及耳蜗毒性的影响。方法选择听力正常的白色红目豚鼠随机分3组,采用听性脑干反应测定,光镜、电镜观察,免疫组织化学及图像分析技术等手段,观察各组动物耳蜗形态学及听功能的变化。结果对照组豚鼠耳蜗结构及听功能无改变。停药后拮抗组与实验组ABR阈值及Ⅰ波潜伏期比较差异有显著性(P<0.01)。显微图像分析结果显示:实验组灰度值较拮抗组小,二者差异有显著性(P<0.01)。结论丹参对顺铂导致的耳蜗毒性具有防护作用,这种作用可能与耳蜗诱导性一氧化氮合酶表达降低有关。

Purpose To explore the effects of salvia miltiorrhiza on cisplatin ototoxicity and investigate the change on the inducible nitric oxide synthase expression. Methods Thirty two white guinea pigs with normal hearing were randomly divided into three groups. Then auditory brainstem response examination, light microscope, immmunohistochemistry, electron microscope and image quantative analysis technique were used to evaluate the change of hearing function and morphology. Results The difference of auditory brainstem response threshold and latrency of wave I between the experimental and the inhibited group was obvious ( P < 0.01). The average grey value in the experimental group was smaller than that in the inhibited ( P < 0.01) . Conclusion Salvia miltiorrhiza has the protective effect on the cisplatin ototoxicity, and this effect may be related to the decreasing expression of inducible nitric oxide synthase.