应用三聚磷酸钠为交联剂制备载药物纳米粒的研究

Study on the preparation of nanoparticles using sodium tripolyphosphate as cross-linking agent

目的 以三聚磷酸钠 (TPP)为交联剂 ,用生物降解聚合物材料壳聚糖 (CS)制成纳米粒 ,研究其对蛋白质的包载和控释性能。方法 壳聚糖是带有阳离子的大分子聚合物 ,与带有阴离子的三聚磷酸钠作用可形成纳米级聚电解质复合物。将聚乙二醇 (PEG)、牛血清白蛋白 (BSA)加入反应体系中即能生成壳聚糖 -聚乙二醇载蛋白质纳米粒。结果 纳米粒的制备条件温和 ,快速简便。纳米粒蛋白质包封率高并可在较长时间范围内连续释放。结论 壳聚糖 -聚乙二醇纳米粒有望成为蛋白质、基因、抗体和药物的输送载体。

Objective:To prepare the nanoparticles with biodegradable polymer chitosan(CS), sodium tripolyphosphate (TPP) was used as cross-linking agent. The character of protein loading and release was investigated.Methods:Chitosan is a cationic polymer, and it can react with the polyanion TPP to produce nanosized polyelectrolytes. Adding polyethylene glycol(PEG) andbovine albumin(BSA) to the reaction solution, CS-PEG nanoparticles loading protein can be prepared.Results:The preparation conditions are mild, fast and simple. The nanopart ic les have a great protein loading capacity and providea continuous releaseof the entrapped protein over a long time.Conclusions:CS-PEG nanoparticle is a very promising vehicle for the delivery of proteins, genes, antigens and drugs.