化学诱导法——卵母细胞去核新策略

Chemical Induction——New Strategy of Oocyte Enucleation

哺乳动物体细胞克隆技术在过去的几年里取得了飞速发展 ,但是核移植的效率依然很低。于是人们不断的从各个方面进行探索 ,去核方法随之也成为其中的一个热点。但是传统的物理去核存在着技术要求高、耗费时间长、对细胞损伤大的缺点 ,作为思路转换的产物 ,一种新的卵母细胞去核技术———Deme诱导去核引起了各国科学家的广泛关注。文章着重介绍了Deme诱导去核辅助的核移植程序 ,Deme诱导去核成功率的影响因素 ,Deme诱导去核方法对卵母细胞及克隆胚胎的影响 ,并结合作者从事的研究对该方法目前存在的问题及某些环节可能的改进措施提出了看法。无论如何 ,Deme诱导去核方法的有效应用仍然需要作进一步的深入研究。

Mammalian somatic cloning has got great progress during past few years,however,the efficiency of nuclear trasfer remains low.In order to improve this technique,different perspectives of which are studying.Accordingly,the method of oocyte enucleation also becomes a focus.But the physical enucleation is technically demanding, time-consuming, inherently invasive and clearly damaging to cytoplast spatial organization. As a alternative strategy to traditional method, Demecolcine-induced enucleation(IE) attracted the scientist's eyes. Nuclear transfer procedure assited with IE,factors releated with success rate of IE and effects of IE on the oocytes and cloned embryos are mainly discussed in this review. At the same time ,combining with author's research, the existing problems of IE and potential improved measures of some key steps in IE procedure are also elucidated. However, the utility of the demecolcine-induced enucleation protocol will require further deep investigation.