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重组人干细胞因子的研究 被引量：2

Study of Recombinant Stem Cell Factor
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摘要 利用基因工程的方法制备了高纯度的人干细胞因子 ,并对制备的重组人干细胞因子的结构和生物活性进行了研究。结果表明rhSCF在磷酸盐缓冲液中以非共价的二聚体形式存在 ,其精确分子量、质量肽谱、氨基酸组成及N端、C段序列等均与理论值一致 ,二硫键位置正确。rhSCF单用或与rhG CSF合用 ,对猴外周血造血祖细胞有明显的动员作用。 Stem cell factor is an important hematopoietic growth factor. In this study, the human stem cell factor was produced by recombinant E.coli,and the structure and biological activity of the recombinant stem cell factor(rhSCF) was studied.It was indicated that the rhSCF was a uncovalent dimer in phosphate buffer,and had the correct mass spectra, mass peptides spectra, composition of amino acid, N-terminal sequernce,C-terminal sequence and intrachain disulfide linkages, rhSCF alone or synergy with rhG-CSF could mobilze hematopoietic progenitors to blood in monkey.
作者 吴军 巩新 唱韶红 赵志虎 左从林 马清钧
机构地区 军事医学科学院生物工程研究所
出处 《生物工程学报》 CAS CSCD 北大核心 2003年第6期698-704,共7页 Chinese Journal of Biotechnology
基金 国家"八六三"高技术重大专项基金资助 (No .Z18 0 3 3 0 )~~
关键词 干细胞因子 基因表达 造血生长因子 结构 生物活性 stem cell factor, gene expression, hematopietic growth factor
分类号 R346 [医药卫生—基础医学]
  • 相关文献

参考文献12

  • 1Williams D E,Eisenman J,Baird A et al.Identification of a ligand for the c-kit proto-oncogene. Cell,1999,63(2):167-174
  • 2Zsebo K M, Wypych J,McNiece I K et al.Identification,purification,and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium. Cell,1990,63(2):195-201
  • 3Huang E,Nocka K,Beier D R et al. The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor,the gene product of the W locus. Cell, 1990,63(2):225-233
  • 4Tsujino Y, Wada H, Misawa M et al.Effects of mast cell growth factor, interleukin-3, and interleukin-6 on human primitive hematopoietic progenitors from bone marrow and cord blood.Exp Hematol, 1993,21(10):1379-1386
  • 5Kamijo T, Koike K, Nakazawa Y et al. Synergism between stem cell factor and granulocyte-macrophage colony-stimulating factor on cell proliferation by induction of cyclins. Cytokine, 2002,19(6):267-275
  • 6Macioce G, Pace A, Ziegler B et al. Unilineage megakaryocytic proliferation and differentiation of purified hematopoietic progenitors in serum-free liquid culture.Blood,1995, 86(10):3725-3736
  • 7van de Ven C,Ishizawa L, Law P et al. IL-11 in combination with SLF and G-CSF or GM-CSF significantly increases expansion of isolated CD34+ cell population from cord blood vs. adult bone marrow. Exp Hematol,1995,23(12):1289-1295
  • 8Stiff P, Gingrich R, Luger S et al. A randomized phase 2 study of PBPC mobilization by stem cell factor and filgrastim in heavily pretreated patients with Hodgkin's disease or non-Hodgkin's lymphoma. Bone MarrTransplant, 2000, 26(5):471-481
  • 9Wadhwa P D, Lazarus H M, Koc O N et al. Hematopoietic recovery after unrelated umbilical cord-blood allogeneic transplantation in adults treated with in vivo stem cell factor (R-MetHuSCF) and filgrastim administration. Leuk Res, 2003,27(3):215-220
  • 10Duarte R F, Franf D A. The synergy between stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF): molecular basis and clinical relevance. Leuk Lymphoma, 2002,43(6):1179-1187

二级参考文献1

  • 1Huang E，Cell，1990年，63卷，2期，2256页

共引文献5

同被引文献21

  • 1郭斯启,奚永志,崔建武,习彩霞,刘楠,梁飞,孔繁华.重组人干细胞因子在原核系统中高效表达的策略[J].中国输血杂志,2004,17(4):227-231. 被引量：3
  • 2吴军,巩新,唱韶红,赵志虎,马清钧.重组人干细胞因子的纯化[J].中国生物化学与分子生物学报,2004,20(6):821-826. 被引量：2
  • 3Williams D E, Eisenman J, Baird A, et al. Identification of a lig-and for the c-kit proto -oncogene[J]. Cell, 1990,63(2):167-174.
  • 4Zsebo K M Wypych J, McNiece I K, et al. Identification,purification,and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium[J]. Cell, 1990,63(2):195- 201.
  • 5Huang E, Nocka K, Beier D R, et al. The hematopoietic growth factor KL is eneDed by the S1 locus and is the ligand of the c-kit receptor, the gene product of the W locus[J]. Cell, 1990,63(2): 225-233.
  • 6Stiff P, Gingrich R, Luger S, et al. A randomized phase 2 study of PBPC mobilization by stem cell factor and filgrastim in heavily pretreated patients with HDgkin's disease or non-HDgkin's lymphoma[J]. Bone Marrow Transplant, 2000,26(5):471-481.
  • 7Wadhwa P D, Lazarus H M, Koc O N, et al. Hematopoietic recovery after unrelated umbilical cord-bloD allogeneic transplantation in adults treated with in vivo stem cell factor(R-MetHuSCF) and filgrastim administration[J]. Leuk Res, 2003,27(3):215-220.
  • 8Duarte R F, Franf D A. The synergy between stem cell factor and granulocyte colony-stimulating factor: molecular basis and clinical relevance[J]. Leuk Lymphoma, 2002,43(6):1179-1187.
  • 9Broudy VC. Stem cell factor and hematopoiesis. Blood, 1997, 90: 1345 - 1364.
  • 10Martin FH, Suggs SV, Langley KE, Lu HS, Ting J, Okino KH, Morris CF, McNiece IK. Primary structure and functional expression of rat and human stem cell facter cDNAs. Cell, 1990, 63 : 203 - 211.

引证文献2

生物工程学报
2003年 第6期

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