摘要
OBJECTIVE: To develop an HPLC method for the simultaneous determination of rhynchophylline, isorhynchophylline,prim-O-glucosylcimifugin and 5-O-methylvisammioside in Babaojingfeng San.METHODS: The chromatograhic separation was performed on C_(18) column(4.6 mm×200 mm, 5 μm), a linear elution of acetonitrile-methanol(1:4) and 0.1% triethylamine solution was adopted at the flow rate of 0.7 mL·min^(-1). The detection wavelength was 254 nm and the column temperature was 30 ℃.RESULTS: Rhynchophylline, isorhynchophylline, prim-Oglucosylcimifugin and 5-O-methylvisammioside had a good linearity at 5.10 to 102.00 μg·mL^(-1)(r=0.9994), 5.95 to 119.00μg·mL^(-1)(r=0.9996), 6.10 to 122.00 μg·mL^(-1)(r=0.9999), 5.65 to113.00 μg·mL^(-1)(r=0.9998), respectively. The average recoveries and RSD were 97.66%(1.32%), 96.72%(0.52%), 98.99%(0.78%) and 99.16%(1.56%), respectively.CONCLUSION: The method was simple, convenience and may be used in the determination of rhynchophylline, isorhynchophylline, prim-O-glucosylcimifugin and 5-O-methylvisammioside in Babaojingfeng San.
OBJECTIVE: To develop an HPLC method for the simultaneous determination of rhynchophylline, isorhynchophylline,prim-O-glucosylcimifugin and 5-O-methylvisammioside in Babaojingfeng San.METHODS: The chromatograhic separation was performed on C_(18) column(4.6 mm×200 mm, 5 μm), a linear elution of acetonitrile-methanol(1:4) and 0.1% triethylamine solution was adopted at the flow rate of 0.7 mL·min^(-1). The detection wavelength was 254 nm and the column temperature was 30 ℃.RESULTS: Rhynchophylline, isorhynchophylline, prim-Oglucosylcimifugin and 5-O-methylvisammioside had a good linearity at 5.10 to 102.00 μg·mL^(-1)(r=0.9994), 5.95 to 119.00μg·mL^(-1)(r=0.9996), 6.10 to 122.00 μg·mL^(-1)(r=0.9999), 5.65 to113.00 μg·mL^(-1)(r=0.9998), respectively. The average recoveries and RSD were 97.66%(1.32%), 96.72%(0.52%), 98.99%(0.78%) and 99.16%(1.56%), respectively.CONCLUSION: The method was simple, convenience and may be used in the determination of rhynchophylline, isorhynchophylline, prim-O-glucosylcimifugin and 5-O-methylvisammioside in Babaojingfeng San.
基金
National Development and Reform Commission of State Engineering Research Center for Innovation Capacity Building projects in 2011(Grant NO.:[2011]1896)
