摘要
The purpose of this study was to detect changes in urine of mice and to clarify the toxicity induced by bupleurotoxin(BETX) using liquid chromatography/quadrupole time-of-flight mass spectrometry(LC-Q-TOF-MS). A procedure for urine analysis using pattern recognition was proposed to evaluate the toxicity induced by BETX in male BALB/c mice. BETX at 2.5 mg/kg was administrated intraperitoneally(i.p.), and urine samples for the metabolomic study were collected from control and BETX experimental groups. Changes in the concentrations of some urine metabolites were detected exclusively in the experimental group. All results suggested that exposure to BETX might cause a disturbance in fatty acid metabolism and the oxidative stress system. These results may not only clarify the underlying mechanism of diverse intoxication effects of BETX but also provide the guidance in preclinical toxicity screening for new drugs.
The purpose of this study was to detect changes in urine of mice and to clarify the toxicity induced by bupleurotoxin(BETX) using liquid chromatography/quadrupole time-of-flight mass spectrometry(LC-Q-TOF-MS). A procedure for urine analysis using pattern recognition was proposed to evaluate the toxicity induced by BETX in male BALB/c mice. BETX at 2.5 mg/kg was administrated intraperitoneally(i.p.), and urine samples for the metabolomic study were collected from control and BETX experimental groups. Changes in the concentrations of some urine metabolites were detected exclusively in the experimental group. All results suggested that exposure to BETX might cause a disturbance in fatty acid metabolism and the oxidative stress system. These results may not only clarify the underlying mechanism of diverse intoxication effects of BETX but also provide the guidance in preclinical toxicity screening for new drugs.
基金
supported by a program of the NCET Foundation,NSFC(81402823)
partially supported by the Global Research Network for Medicinal Plants(GRNMP)
by King Saud University,Shanghai Leading Academic Discipline Project(B906)
FP7-PEOPLE-IRSES-2008(TCMCANCER Project 230232)
the Key laboratory of drug research for special environments,PLA
the Shanghai Engineering Research Center for the Preparation of Bioactive Natural Products(10DZ2251300)
the Scientific Foundation of Shanghai China(09DZ1975700,09DZ1971500,10DZ1971700)
the National Major Project of China(2011ZX09307-002-03)
the National Key Technology R&D Program of China(2012BAI29B06)
