日本纺织娘种群的RAPD多态性研究(直翅目:螽斯总科:纺织娘科)

RAPD study of Mecopoda. nipoensis (Orthoptera: Tettigonioidea) from seven location sites

采用RAPD技术,从42条引物中筛选出S5,S45,S48,S142等4条引物,对采自甘肃碧口、四川绵阳、四川竹峪、广西猫儿山、广西百色、广西北海、广西弄岗国家级自然保护区等7个种群共38个日本纺织娘(Mecopoda.nipoensisHaan.)个体进行了RAPD PCR扩增,以探讨该种不同种群间的遗传关系.聚类分析结果表明,7个日本纺织娘的研究种群可以分为2组;广西的4个种群为一组,其中百色和弄岗种群的遗传距离较近;其余的3个种群为另一组,其中绵阳和竹峪种群的遗传距离较近.

The 41 samples of Mecopoda.nipoensis (belonging to Mecopodidae) from 7 location sites are analyzed for genetic polymorphisms using RAPDPCR, the clustering result showed that this 7 populations should be divided into 2 groups, one includes 4 populations in Guangxi province, and the other includes the rest 3 populations.