摘要
20 0 2年 4～ 8月 ,在中国科学院海北高寒草甸生态系统定位站对高原鼠兔的种群进行标志重捕 ,用眶下取血法对标志的鼠兔各取血样 10 0 μL ,使用 8对引物对血样中的DNA微卫星序列进行扩增。结果每对引物所扩增的产物都具有明显的多态性 ;结合野外行为观察对这些多态性位点进行分析 ,基本上对当年出生幼体的父权进行了准确的确定 ;从中发现高原鼠兔的交配体制为多配制 ,其家庭中存在等级制度 ,处于统治地位的雄性的繁殖成功率明显高于处于从属地位的雄性个体。上述工作表明微卫星技术在动物行为研究中具有很高的优越性 ,在高原鼠兔社会动态、亲缘关系和行为进化等研究领域具有广阔的应用前景。
From April to August of 2002,we researched the reproductive behaviors of plateau pikas (Ochotona curzoniae) in the Haibei Research Station of Alpine Meadow Ecosystem by mark-recapture method and microsatellite technology.Blood samples (each 100?μL) of the marked plateau pikas were collected from suborbital vein.Eight polymorphic microsatellite loci were amplified with these blood samples.Results showed that all loci were polymorphic.We identified the kinship of marked individuals by analyzing the polymorphic microsatellite loci combining with the animal's behavior,and we found that there existed monogamy,polygamy and promiscuity in the mating system and a hierarchism at the family of the plateau pika.The reproductive success of dominance males was higher than that of subordinate ones.Those indicated that microsatellite technology has an obvious superiority in the field research and is a useful method in the research of plateau pika's society dynamic,kinship and behavior evolution.
基金
国家自然科学基金资助项目 ( 3 9770 10 6
3 0 2 70 2 0 0 )
中国科学院知识创新工程资助项目 (KSCX2 -1-0 3
KSCX2 -SW -10 3 )
关键词
高原鼠兔
微卫星
多态性
亲缘关系
交配制度
Ochotona curzoniae
Microsatellite
Polymrophic
Kinship
Mating system
