高效液相色谱法测定淫羊藿中淫羊藿定C和淫羊藿苷的含量

Determination of Epimedin C and Icariin in Herba Epimedii by HPLC

目的 :测定不同种和不同产地的淫羊藿中淫羊藿定C和淫羊藿苷的含量 ,探讨淫羊藿药材的质量控制方法。方法 :色谱柱为HypersilBDS C18(4mm× 2 50mm ,5μm ) ,以乙腈 0 .0 5%磷酸作为梯度洗脱流动相 ,G 13 15A光电二极管阵列检测器 ,检测波长 2 72nm。结果 :淫羊藿定C和淫羊藿苷平均回收率分别为 99.7% ,10 2 .5% ,RSD分别为 1.5% ,1.1%。结论 :本方法快速 ,重现性和稳定性好 。

Objective:To establish a RP-HPLC metho d for the determination of e pimedin C and icariin in Herba Epimedii. Method:Chromato graphic conditions: Hy persil BDS-C 18 column, acetonitrice and water containing 0.05% phosphori c acid a s gradient eluents, G1315A photodiode array detector at 272 nm. Resu lt:The ave rage recovery rate of epimedin C was 99.7%, RSD1.5% (n=9), icariin 102.5%, RSD1.1 % (n=9). Conclusion:The method is reliable, stable a nd well repeatable. It provides the useful information for quality evaluation.