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细菌人工染色体文库的构建与鉴定 被引量：2

Construction and Identification of Bacterial Artificial Chromosome Library, a Review
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摘要 细菌人工染色体是继 λ噬菌体、黏粒、噬菌体人工染色体、酵母人工染色体等载体系统之后发展起来的 DNA载体系统 ,以其容量大、遗传特性稳定、嵌合体少、插入片段易回收、操作简便等优点 ,而被广泛应用于基因组较大的真核生物基因组研究中 ,并发挥着前所未有的重要作用。因此本文概括性地阐述了细菌人工染色体的发展 ,以及利用此载体构建基因组文库的机理和程序及其鉴定方法。 The construction of DNA vector systems such asλ bacteriophage,cosmid,P1-derived artificial chromosome,yeast artificial chromosome , bacterial artificial chromosome(BAC)system has been developed as another powerful DNA vector system. Being capable of inserting large fragments, maintaining the stability of inserted DNA in E.coli,producing few cherisms,recovering inserted DNA from E. coli cells,and being easy in library construction,BAC is being widely used and producing unprecedented effect in the research of larger eukaryote genomes. This paper presents a brief introduction to the development of BAC,and the mechanism and process of BAC library construction and identification.
作者 周向梅 关伟军
机构地区 中国农业大学动物医学院 中国农业科学院畜牧研究所
出处 《中国农业科技导报》 CAS CSCD 2003年第6期40-43,共4页 Journal of Agricultural Science and Technology
关键词 细菌人工染色体 文库构建 鉴定技术 DNA载体系统 容量 遗传特性 真核生物基因组 bacterial artificial chromosome BAC library construction identification
分类号 S188 [农业科学—农业基础科学]
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参考文献18

  • 1[1]Cai Li, Taylor J. F. , Wing R.A. et al. Construction and Characterization of a Bovine Bacterial Artificial Chromosome Library [J]. Genomics, 1995,29:413-425
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同被引文献41

  • 1Shizuya H,Birren B,Kim U J,et al.Cloing and Stable Maintenance of 300-kilobase-Pair Fragments of Human DNA in E.coli using an F-factor-based vector[J].Proc Natl Acad Sci USA,1992,89:8794～8797.
  • 2Texas A&M BAC Center.The BAC LIBRARY PROTOCOLS (1)(2)(3).2000.1～35.
  • 3SANGDUN CHOI,ROD A.WING.The construction of bacterial artificial chromosome (BAC) libraries.CUGI Protocols.1999.1～26.
  • 4Davis D L,Nielsen M T.烟草-生产,化学和技术.北京:化学工业出版社,2002.65～80.
  • 5Ozdemir N,Horn R,Friedt W.Isolation of HMW DNA from sunflower (Helianthus annuus L.) for BAC cloning.Plant Molecular Biology Reporter,2002,20:239～250.
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  • 7BURKE D T,CARLE G F,OLSON M V. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors[J].Science,1987,(4803):806-812.
  • 8BELLIS M,GéRARD A,CHARLIEU J P. Construction and characterization of a partial library of yeast artificial chromosomes from human chromosome 21[J].DNA Ceil Biol,1991,(04):301-310.
  • 9GREEN E D,RIETHMAN H C,DUTCHIK J E. Detection and characterization Of chimeric yeast artificial-chromosome clones[J].Genomics,1991,(03):658-669.
  • 10SHIZUYA H,BIRREN B,KIM U J. Cloning and stable maintenance of 300-kilo base-pair fragments of human DNA in Eschericha coli using an F-factor-based vector[J].Proceedings of the National Academy of Sciences(USA),1992,(18):8794-8797.

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中国农业科技导报
2003年 第6期

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