小型化无透镜微流控片上生物成像检测

Miniaturized lensless microfluidic on-chip bio-imaging detection

用于生物医疗成像的传统高分辨率光学显微镜由于复杂的光学透镜系统导致高成本、不便携。为了实现小型化成像检测,基于CMOS图像传感芯片与微流控集成的无透镜微流控片上成像系统近年来发展迅速,是解决小型化显微成像检测的重要方法。综述了无透镜微流控片上成像系统的结构、成像原理、存在的问题与改进的方法,主要包括阴影成像系统、全息成像系统、荧光成像系统以及彩色成像系统等,分析了无透镜微流控片上成像系统的不足和可能的发展方向。

The conventional high-resolution optical microscopy for biomedical imaging has high cost and low portability due to bulky optical lenses system.To realize miniaturized imaging,the recent advance of lensless microfluidic on-chip imaging technique based on the integration of CMOS image sensor and microfluidics has provided one promising solution.Therefore,the architecture,principle,problems and improvement solutions for different miniaturized lensless microfluidic on-chip bio-imaging,including shadow imaging,holographic imaging,fluorescent imaging and color imaging were reviewed.And the deficiencies and possible development directions were discussed.