含吡咯里西啶生物碱的山紫菀类药材的分子鉴别

Molecular Identification of Ligularia Herbs Containing Hepatoto xic Pyrrolizidine Alkaloids

目的 :采用分子生物学的方法对含吡咯里西啶生物碱 (HPAs)与不含吡咯里西啶生物碱的山紫菀类药材 (橐吾属植物 )进行鉴别。方法 :采用CTAB法提取 9种不同基源的山紫菀类药材的总DNA ,进行PCR扩增和测序 ,并对序列进行系统分析。结果 :通过提取、扩增和测序 ,得到了 9种橐吾属植物的nrDNAITS区间序列 ,统计分析结果得到了 9种植物的遗传距离和转换、颠换数 ,分支分类结果得到了系统树。结论 :9种橐吾属植物nrD NAITS区间序列的分析结果表明了两类山紫菀类药材有着明显的区别 。

AIM:Some plants from genus Ligularia are m edicinal herbs, named “Shanziwan” in Chinese, but some of them contain hepatot oxic pyrrolizidine alkaloids (HPAs). The aim of this paper is to establish a m o lecular method to distinguish the HPAs-containing Ligularia herbs from the others. METHOD:The geno m e DNAs from nine specis of Ligularia were extracted by CTAB method, amplifi ed by PCR method and then sequenced. The obtained data were analyzed by Clustal X 1 . 8 and Mega V 2.1 software. RESULT:The obtained nrDNA ITS sequence s of nine speci s of Ligularia were analyzed by statistics method to give the genetic distan ces, numbers of transition and transversion, by maximum parsimony method to give the phylogenetic tree. CONCLUSION:The analytical results of n rDNA ITS sequences of nine Ligularia plants showed that there were obvious differences between HPAs-containing and no-HPAs herbs.