鱼类细胞的旋转管培养法

Rotation culture of fish cells

用圆柱形细胞培养瓶,对草鱼吻端组织ZC—7901细胞适应旋转培养条件进行了研究,测定了细胞接种浓度、旋转速度及细胞生长速度等有关参数。试验表明ZC—7901细胞能适应于旋转培养,适应后的细胞对草鱼出血病病毒仍具有敏感性。

A new method was developed for rotation culture of ZC-7901 cells, a cell line from grass carp snout. The culture condition such as cell inoculation concentration, rotation speed and cell growth rate were studied. The results showed that ZC- 7901 cells was suitable for rotation culture. The cells grown in rotation bottle were still susceptible to grass carp hemorrhage virus.