1,10-二氮杂菲固相萃取光度法测定水中的铁

Study on solid phase extraction spectrophotometry for determination of iron in water with 1,10-phenanthroline

研究了1,10 二氮杂菲与铁的显色反应,在pH4 5的盐酸-六次甲基四胺缓冲介质中,1,10 二氮杂菲与铁反应生成3∶1稳定络合物,该络合物可被C18固相萃取小柱萃取,小柱上富集的络合物用乙醇洗脱后用光度法测定,λmax=510nm,ε=1 03×104。铁含量在0～4μg/mL内符合比尔定律,方法用于饮用水中铁含量的测定,结果满意。

The color reaction of iron with 1,10phenanthroline has been studiedIn the presence of pH 45 HCl-(CH2)6N4 buffer solution,1,10phenanthroline can react with iron*' to form a stable 3∶1 complexThis complex can extracted by Waters Plus-C18 column and eluted by ethanolThe apparent molar absorption coefficient is 103×104L·mol-1·cm-1 at 510nmBeer's law is obeyed in the range of 0-4μg/mLThis method has been applied to the determination of iron in drinking water with satisfactory results