人脑胶质瘤中cyclin D2的表达及其与细胞增殖的关系

Expression of cyclin D2 and its relationship with cellular proliferation in human brain gliomas

目的 :研究人脑胶质瘤中cyclinD2蛋白的表达与肿瘤恶性程度的关系及其对细胞增殖活性的影响 .方法 :采用免疫组化方法检测 5 2例人脑胶质瘤和 8例正常人脑组织标本中cyclinD2和PCNA的表达水平 ,计算两者标记指数 ,统计分析两者之间及其与胶质瘤病理分级之间的关系 .结果 :正常脑组织中无cyclinD2或PCNA表达 .随着人脑胶质瘤病理分级的增高 ,cyclinD2平均标记指数明显升高 .在WHO分级Ⅰ～Ⅳ级胶质瘤中 ,Ⅰ ,Ⅱ ,Ⅲ级与Ⅳ级、Ⅰ级与Ⅲ级以及低度恶性胶质瘤 (Ⅰ～Ⅱ )与高度恶性胶质瘤 (Ⅲ～Ⅳ )之间标记指数存在显著性差异 (P <0 .0 5 ) .类似cyclinD2 ,PCNA标记指数随脑胶质瘤恶性程度增高而升高 .cyclinD2与PCNA的平均标记指数呈明显正相关 (r =0 .6 5 4 ,P <0 .0 1 ) .结论 :人脑胶质瘤中cyclinD2的表达与细胞增殖活性和病理学分级密切相关 ,很可能参与了人脑胶质瘤细胞的恶性转化 .

AIM: To study the expression of cyclin D2 in human brain gliomas and its relationship with pathological grades and cellular proliferation activity. METHODS: The expression of cyclin D2 and PCNA was detected by immunohistochemistry in 52 human brain gliomas and 8 normal brain tissues. The positive rates and the labeling indices of cyclin D2 and PCNA were counted and compared with the pathological grades of gliomas, respectively. RESULTS: Neither cyclin D2 nor PCNA was expressed in normal brain tissues. With the advancing of pathological grades, the positive rates and mean labeling indices for cyclin D2 in human brain gliomas increased accordingly. Significant difference was seen in labeling indices between grade Ⅰ, Ⅱ, Ⅲ and Ⅳ ( P <0.05) as well as between grade Ⅰ and Ⅲ ( P <0.01). Remarkable difference between low grade (grade Ⅰ and Ⅱ) and high grade (grade Ⅲ and Ⅳ) was also observed in both two indices ( P <0.05 ). Similar to cyclin D2, PCNA labeling indices increased with the malignancy grades of brain gliomas. A positive correlation was seen between the labeling indices for cyclinD2 and PCNA( r= 0.654, P <0.01). CONCLUSION: There is a positive correlation between the expression of the cyclin D2 and the cellular proliferation activity as well as pathological grades in human brain gliomas. Cyclin D2 may be involved in the progression of human brain gliomas.