cAMP类似物对人乳腺癌细胞cyclinD1表达的影响

Effect of cAMP analogs on cyclinD1 expression of human breast cancer cells

目的 探讨cAMP类似物对人乳腺癌细胞增殖相关cyclinD1基因表达的影响。方法 体外分组原代培养人乳腺癌细胞 ,采用原位杂交方法研究cAMP类似物对人乳腺癌细胞中cyclinD1基因表达的变化。 结果 8 Br cAMP、8 Cl cAMP均下调乳腺癌细胞中cyclinD1基因表达 ,且后者下降作用更明显。

AIM To study the effect of cAMP analogs on cyclinD1, expression of human breast cancer cells. METHODS After the human breast cancer cells were cultured, the alteration of cyclinD1 gene expression was studied by in situ hybridization. RESULTS 8 Br cAMP, 8 Cl cAMP could reduce the expression of cyclinD1, the effect of 8 Cl cAMP was stronger than that of 8 Br cAMP. CONCLUSION cAMP analogs could inhibit the grouth of human breast cancer celols by affecting the cyclinD1 gene expression.