中药红毛五加化学成分的高效液相色谱/电喷雾电离/质谱/质谱(HPLC/ESI/MS2)分析

High Performance Liquid Chromatography/Electrospray Ionization/Mass Spectrometry^2 Analysis of Acanthopanax giraldii Harms

建立了中药红毛五加化学成分的HPLC/ESI/MS2定性分析方法。液相色谱条件:ZorbaxSB C18色谱柱(250mm×4 6mmi d ,5μm);乙腈 水(用冰醋酸调pH为3 0)梯度洗脱,流量0 8mL/min;柱温30℃;二极管阵列检测器(DAD),检测波长254nm。质谱条件:Agilent离子阱质谱仪;电喷雾离子(ESI)源;正离子检出模式。采用该方法得到了红毛五加的紫外(UV)色谱图、总离子流色谱图(TIC)和萃取离子色谱图(EIC),以及相应色谱峰的EIC/MS2的质谱图,对其进行解析,鉴别出红毛五加中的鸟苷、腺苷和紫丁香树脂苷成分。方法简便、快捷和准确。

A high performance liquid chromatography/electrospray ionization/mass spectrometry/mass spectrometry (HPLC/ESI/MS2) method has been developed for the qualitative analysis of Acanthopanax giraldii Harms. The separation was performed on Zorbax SBC18 column (250 mm×46 mm id, 5 μm) with gradient elution of acetonitrilewater (the pH was adjusted to 30 with acetic acid) as mobile phase with a flow rate of 08 mL/min at 30 ℃. The photodiode array detector (DAD) was set at 254 nm. The MS analysis was carried out on an Agilent 1100 series ion trap mass spectrometer with an electrospray ionization source in the positive ion mode. The ultraviolet (UV) chromatogram, total ion current chromatogram (TIC) and extracted ion chromatogram (EIC) of the sample and ESI/MS2 spectra of the peaks in the chromatograms were obtained. Guanosine, adenosine and liriodendrin in the Acanthopanax giraldii Harms were identified. This method is easy to run, fast and accurate.