高效液相色谱/二极管阵列检测/质谱/质谱(HPLC/DAD/MS^2)联用在板蓝根注射液成分鉴定中的应用

High Performance Liquid Chromatography/Photodiode Array Detection/Mass Spectrometry^2 (HPLC/DAD/MS^2) Identification of Ingredients of Banlangen Injection

用HPLC/DAD/MS2联用仪,鉴定了板蓝根注射液中含有的核苷类物质。色谱条件:LunaC18色谱柱(250mm×4 6mmi d ,5μm);甲醇 水梯度洗脱,流量0 8mL/min;柱温25℃。用二极管阵列检测器记录各个色谱峰的紫外吸收光谱,色谱检测波长254nm。质谱条件:电喷雾离子源(ESI);正离子检测;扫描范围m/z50～800。记录质量色谱图和各个色谱峰的一级、二级质谱图,并对质谱结果进行解析,通过与对照品比较,确定板蓝根注射液样品中含有腺苷、鸟苷、尿苷、胞苷及腺嘌呤等5种成分。

The determination of the ingredients of Banlangen (a Chinese traditional medicine) injection has been carried out by HPLC/DAD/MS2. The separation was performed on a Luna C18 column (250 mm×46 mm id, 5 μm) with gradient elution of watermethanol as mobile phase with a flow rate of 08 mL/min at 25 ℃. The detection wavelength was 254 nm and the UV spectra of peaks were obtained by a photodiode array detector (DAD). The electrospray ionization(ESI) source and positive scan mode were used to get mass chromatogram, MS and MS2 spectra. The Banlangen injection ingredients of adenine, guanosine, uridine, cytidine and adenosine were identified.