应用RT-nPCR检测石蜡包埋甲状腺癌中PTC癌基因的表达

Detection of PTC Oncogene in Human Thyroid Carcinoma by RT-PCR

为探讨ret原癌基因活化形式—PTC癌基因在甲状腺癌发病机制中的作用和判断其生物学行为,运用RT-nPCR技术检测16例甲状腺乳头状癌、4例滤泡癌、2例未分化癌、1例髓样癌PTC癌基因mRNA的表达,结果表明:16例甲状腺乳头状癌中有7例(43％)PTC基因表达为阳性,甲状腺滤泡癌、髓样癌及未分化癌均为阴性。在甲状腺乳头状癌中,PTC表达阳性组多伴有淋巴细胞浸润(57％),高于阴性组病侧(11％)(P<0.05),而与患者发病年龄、肿瘤大小及淋巴结转移无显著相关性。因此,我们认为ret原癌基因的活化与甲状腺乳头状癌的发生有关,其活化形式—PTC癌基因的表达仅限于乳头状癌,可作为甲状腺乳头状癌的诊断和鉴别诊断的有效参考指标。

To investigate the frequency and significance of ret oncogene activiation in human thyroid carcinomas, expression of PTC oncogene were detected in paraffin embedded tissue soecimens from 23 thyroid carcinomas by RT-PCR, which included 16 case papillary carcinomas,4 case follicular carcinomas, 2 case undifferential carcinomas, 1 cases medullary carcinoma. PTC oncogene was expressed in 7 of 16 case(43% ) papillary carcinomas,by contrast, none of follicular carcinomas, undifferential carcinomas, medullary carcinoma showed positive. The frenquency of lymphocytic infiltration with tumors displaying PTC oncogene was significantly higher (57 %) than the cases that did not present PTC oncogene (11%). No significant differences were found regarding the gender of the patient, tumor size or the lymph node metastasis. We suggest that PTC oncogene be unique to papillary carcinoma subtype. It may be play an important role in the oncogenesis of papillary carcinoma and is useful in diagnosis of thyroid carcinoma.