抗酒石酸酸性磷酸酶测定及在癌症骨转移诊断中的应用

Determination of Tartrate-resistant acid phosphatase and its application in cancer metastases to bone

目的 建立抗酒石酸酸性磷酸酶测定方法及在癌症骨转移诊断中的临床应用。方法 以 1-萘酚磷酸为基质 ,测定肝素抗凝血抗酒石酸酸性磷酸酶。结果 抗酒石酸酸性磷酸酶测定方法的参考值为 2 .6 5 .0U L ,该方法的平均批内和批间变异系数分别为 2 .85 %和 5 .4 5 % ,线性范围为 1 35U L。癌症骨转移患者该酶活性明显高于对照组 (P <0 .0 0 1)。结论 抗酒石酸酸性磷酸酶测定方法简便 ,快速 ,可作为癌症骨转移患者的诊断和疗效观察的辅助检验指标。

Objective To establish a method for examining tartrate-resistant acid phosphatase(TrACP) and its application in cancer metastases to bone.Methods To measure the activity of tartrate-resistant and fluoride-sensitive acid phosphatase by using 1-naphthol phosphate as substrate.Results The reference range of this method was 2.6-5.0 U/L. The CV of this method was averaged 2.85%(within-run)and 5.45%(between-run), respectively. The linearity of method was 1-35 U/L. The activity of TrACP in cancer metastases to bone was significantly higher than control group (P<0.001).Conclusion TrACP was a simple and rapid method for diagnosing and monitoring of cancer metastases to bone.