系统性红斑狼疮患者外周血中性粒细胞CD89表达在发病中的意义及免疫调节治疗对其影响

The effect of CD89 expression of peripheral neutrophiles in the pathogenesis and immuno regulatory therapy of systemic lupus erythematosus

目的 本研究旨在探讨系统性红斑狼疮伴有血尿的患者应用免疫调节治疗前后外周血中性粒细胞CD89表达水平及血尿的变化在发病机理中的作用及免疫调节治疗对其影响。方法 应用直接免疫荧光法流式细胞术 ,检测 2 0例健康志愿者及 2 0例系统性红斑狼疮伴有血尿的患者外周血中性粒细胞CD89的表达情况 ,并分析其与血尿的关系及免疫调节治疗对CD89表达的影响。结果 系统性红斑狼疮伴有血尿的患者其外周血中性粒细胞CD89的表达水平明显低于健康对照组 (P <0 .0 1)。治疗一个月后 ,CD89表达水平上升 ,血尿亦减轻。相关分析显示 ,系统性红斑狼疮伴有血尿的患者血尿定性检测结果与患者外周血中性粒细胞CD89表达水平呈明显负相关 ,治疗前后相关系数分别为r =- 0 .5 82 1(P <0 .0 1)和r =- 0 .794 8(P <0 .0 1)。结论 系统性红斑狼疮伴有血尿的患者外周血中性粒细胞CD89表达水平明显降低 ,免疫调节治疗可使其上升。患者血尿的发生与中性粒细胞CD89表达的减少有关。外周血中性粒细胞CD89表达缺陷可能是引起系统性红斑狼疮血尿的原因之一。

Objective To determine the CD89 levels of peripheral neutrophiles in systemic lupus erythematosus (SLE) patients with hematuria before and after immuoregulatory therapy for evaluating the relationship between CD89 level and the formation of renal hematuria, as well as to investigate the pathogenesis of hematuria in SLE.Methods Applying direct immunofluorescence method and flow cytometry to determine CD89 levels of peripheral neutrophiles of 20 healthy volunteers and 20 SLE patients before and after therapy.Results Compared with health controls, CD89 levels of peripheral neutrophiles in SLE were decreased (P<0.01). CD89 levels were elevated after therapy for 1 month. It's revealed that CD89 levels were negatively correlated with the extent of hematuria of SLE either before (r=-0.5821) or after (r=-0.7948) therapy.Conclusion CD89 levels of peripheral neutrophiles in SLE were decreased. CD89 levels were elevated and hematuria were decreased after immunoregulatory therapy. The decreased CD89 level may play an important role in the pathogenesis of hematuria in SLE.