高同型半胱氨酸致脑血管疾病的作用机制初步探讨

Investigatigating of mechanism of hyperhomocysteinemia leading to cerebrovascular disease

目的 探讨脑卒中和暂时性脑缺血 (TIA)患者血浆同型半胱氨酸水平 (HCY)与脂质过氧化反应之间的关系 ,进一步揭示高同型半胱氨酸致动脉粥样硬化的作用机制。方法 我们采用荧光偏振免疫分析和硫代巴比妥酸的方法分别对 77例脑卒中患者 ,2 8例TIA患者和 2 0例健康查体者 (对照组 )的血浆HCY和丙二醛 (MDA)水平进行了检测。结果 脑卒中患者血浆中HCY和MDA水平与对照组比较有显著性差异 (P<0 0 5 ) ,其血浆HCY水平与MDA水平呈正相关 (r =0 2 91)。TIA患者组血浆中HCY和MDA水平与对照组比较未见显著性差异 (P >0 0 5 ) ,其血浆中HCY水平与MDA水平比较未见相关 (r =0 10 9)。结论 高HCY可能引起机体的氧化应激反应 ,产生超氧化阴离子自由基O 2 和H2 O2 。此过程在暂时性脑缺血情况作用不明显 ,而在脑卒中的发病中可能起到一定的作用。

Objective To observe the relationship between plasam levels of homocysteine(HCY)and oxidant stress in subjects with stroke or ransient ischemic attack(TIA).Methods we assayed HCY and Malondiadehyde(MDA)in samples from 77 cases with stroke.28 cases with TIA and 20 control cases.Results In cases with stroke.The plasma level of HCY and MDA were significantly higher than those in control group,and there was significant correlation between the plasma level of HCY and MDA.In cases with TIA,the HCY level was slightly higher than that in control group.but the difference was statistically nonsignificant.The MDA level did not differe from that in control group significantly.and there was nonsignificant association between the elevated plasma HCY and MDA.Conclusion Oxidative stress induced by hyperhomocysteinemia which it is an important role in stroke and nonsignificent in TIA.