小儿肺炎支原体感染的检验分析

Analysis of Test of Mycoplasma Pneumoniae Infection in Children

目的分析小儿肺炎支原体感染(Mycoplasma pneumoniae infection,MPI)的检验结果。方法为提高小儿MPI诊断准确率提供参考;选择2015年1月—2016年12月于该院接受诊断的MPI患儿118例,以被动凝聚法和金标法检测所有患儿肺炎支原体总抗体(IgG+IgM+IgA)及IgM抗体,检测严格依据试剂盒要求进行。同时并进行痰及细菌培养、血常规、尿常规、冷凝集试验、血生化等实验室检查。结果血清IgG+IgM+IgA及IgM抗体、痰及细菌培养、血常规、尿常规、冷凝集试验、血生化等检测诊断小儿MPI的特异性均相对较低。结论单项实验室检测项目诊断小儿MPI缺乏特异性,故在小儿MPI诊断中,需对患儿进行血清IgG+IgM+IgA及IgM抗体、痰及细菌培养、血常规、尿常规、冷凝集试验、血生化等实验室检查,并对各种检查结果进行综合分析,在提高诊断准确率的同时,避免漏诊、误诊,为小儿MPI的治疗提供重要参考。

Objective To analyze the test results of Mycoplasma pneumoniae infection in children and provide reference for the MPI diagnosis accuracy rate in children. Methods 118 cases of MPI children treated in our hospital from January 2015 to December 2016 were selected and the IgG+IgM+IgA and IgM antibody of all children were tested by the forced coacervation method and golden-standard method, and the test should be strictly base on the kit requirement, and the sputum and bacterial culture, routine blood test, routine urine test, cold agglutination test and blood biochemistry were conducted. Results The specificity of serum IgG +IgM +IgA and IgM antibody, sputum and bacterial culture, routine blood test, routine urine test, cold agglutination test and blood biochemistry in diagnosis of MPI in children was relatively low. Conclusion The single laboratory test items in diagnosis of MPI in children lacked specificity, therefore, in the diagnosis of MPI in children,we need to conduct the laboratory examinations of IgG+IgM+IgA, IgM antibody, sputum and bacterial culture, routine blood test, routine urine test, cold agglutination test and blood biochemistry and conduct the overall analysis of various examination results thus improving the diagnosis accurate rate, avoiding the missed diagnosis and misdiagnosis and providing important reference for the treatment of MPI in children.