凝集素在食源致病菌快速检测中应用的研究进展

Progress in application of lectin in rapid detection of food-borne pathogens

凝集素是一类具有独特糖专一性、能够与糖非共价可逆结合的蛋白质。食源致病菌表面存在大量糖蛋白分子,凝集素因能够与其发生高亲和力的结合,被广泛应用于食源致病菌的快速检测中。凝集素作为识别分子用于食源致病菌的分离与检测,能够改善检测新方法的实用性、消除食品基质的干扰、缩短样品的处理时间、提高检测的灵敏度。本文介绍了凝集素的基本信息及其糖特异性识别机制,并对凝集素在食源致病菌快速检测领域的应用进展进行了综述。

Lectin can bind with carbohydrate specifically,non-covalently and reversibly,known as a kind of carbohydrate-binding proteins.The presence of a large number of glycoprotein molecules on the surface of food-borne pathogens makes it possible that lectin bind with food-borne pathogens with high affinity,making lectin been widely used in the rapid detection of food-borne pathogens.When applied as a recognition agent for the separation and detection of food-borne pathogens,lectin harbors the merits of improving the practicability of the new detection methods,eliminating matrix interference,improving sensitivity and shortening sample processing time.In this article,the basic information of the lectins and the mechanism of carbohydrate specificity recognition were introduced,and the application progress of lectin used in the field of rapid detection of food-borne pathogens were reviewed.