邻苯二酚1,2-双加氧酶的结构、功能及同工酶现象研究进展

Advances in study on structure, enzymatic property and isoenzymes of catechol 1,2-dioxygenases

邻苯二酚是芳香族化合物多条生物降解途径中共有的一种重要的中间产物,根据开环方式的不同,可分为邻位降解途径和间位降解途径,其中邻位降解途径中的关键酶是邻苯二酚1,2-双加氧酶。本文主要综述了邻苯二酚1,2-双加氧酶的结构、酶学性质,以及它在芳香烃降解菌中存在的同工酶现象及其功能研究进展。

Catechol is one of the most important intermediate products in the various pathways in degrading polycyclic aromatic hydrocarbons(PAHs).The ring cleavage can occur in two different orientations relative to the vicinal diols,and this difference in cleavage site is typically used to classify the catechol dioxygenases into two groups,the intradiol-and extradiol-cleaving enzymes.The essential process of intradiol pathway is the intradiol ring-cleavage catalyzed by catechol 1,2-dioxygenases.This paper summarized the research advances in these fields with the focus on the structure,enzymatic property and isoenzymes of the catechol 1,2-dioxygenases.