影响麻疹病毒血凝素滴度的某些因素的探讨

STUDIES ON SEVERAL FACTORS AFFECTING THE HEMAGGLUTININ TITER OF MEASLES VIRUS

自从Peries等报告麻疹病毒具有凝集猴血球的特性之后,近来有不少作者证实了这一研究。但麻疹病毒的血凝滴度较低,作血凝抑制试验时,一般均采用理化方法将血凝素加以浓缩。我们曾对麻疹病毒的血凝性质进行研究,可以不经浓缩而获得滴度较高的血凝素。关于应用麻疹病毒血凝抑制试验检查麻疹抗体的研究,已有报告。现将影响麻疹病毒血凝素滴度的某些因素报告如下:

A study on several factors affecting the hemagglutinin titer of measles virus wasmade. Without concentrating the infectious tissue culture fluid, the hemagglutinating titreof 1:32--1:64 has been obtained. The following factors were found to be important inobtaining high titre hemagglutinin: 1). Type of cell culture used--continuous cell linewas better than its corresponding primary cell culture and human renal cell culture betterthan human ammion cell culture; 2). With the same type of cells, cultures showing over-rapid development of C P E gave low titre of hemagglutinin, although the infectivitytitre was found to be approximately the same as those giving high titre of hemagglutininwith late development of C P E culture. Change of maintenance medium after 5--6 daysof infection in primary cell culture or aftec 2--3 days in continuous cell lines not onlymaintained the cell in good condition but also stimulated the rapid development ofCPE. Thus leading to the production of high titre hemagglutinin; 3). Cell cultures in-cubated at 33℃ gave a better production of hemagglutinin than those incubated at 37℃;4). Treatment of infected cell culture with ultrasonic wave increased the yield of hemag-glutinin to 4 folds. By decreasing the concentraction of red blood cell from 1% to0.5%, the hemagglutinin titre could be raised to 2 folds.