摘要
多粘杆菌（Bacillus polymyxa）AS1.546产β-淀粉酶的最适培养基成份（%）为:玉米粉4,豆饼粉2,酵母膏0.5,Na2HPO4·12H2O0.2,NaH2PO4·2H2O0.03,MgSO4·7H2O0.05,自然 pH（7左右）。250毫升三角瓶装50毫升培养基,旋转式摇床（220转/分）,温度为30℃,培养时间为60小时,每毫升发酵液酶活力可达700单位。酶反应的最适温度为45℃,最适pH 为6.5,水解可溶性淀粉生成麦芽糖可达96%。
Bacillus polymyxa AS 1.546 wasselected from 300 strains of microorganismsas a β-amylase producer.The suitable me-dium composition （%） was:corn meal 4,soybean cake meal 2,yeast extract 0.5,Na2HPO4·12H2O 0.2,NaH2PO4 0.03;MgSO4·7H2O 0.05.When the organismwas grown in 250 ml flasks containing 50ml of the above medium on a rotaty shaker（220 rpm） at 30℃ for 60 hours,700 unitsof β-amylase per ml of culture broth wasobtained.Under optimal conditions （pH6.5,45℃）,The enzyme converted starchto maltose with a yield of 96%.
出处
《微生物学报》
CAS
1980年第4期421-426,共6页
Acta Microbiologica Sinica
