流行性感冒病毒重组的研究——Ⅰ.高产株和表面抗原重组株的选育

STUDIES ON RECOMBINATION OF INFLUENZA VIRUSES——Ⅰ.SELECTION OF HIGH YIELDING AND SURFACE ANTIGEN RECOMBINANTS

本文报告了甲型流感病毒重组高产株、血凝素单价抗原重组株及神经氨酸酶单价抗原重组株的选育和鉴定方法。实验证明利用灭活病毒和活病毒两株亲本株混合感染,在鸡胚系统中重组,经过抗血清中和选择,再经终末纯化,可以简便、快速而又准确地获得重组株。

The present work describes the pro-cedures for recombination,selection andidentification of a high yielding recom-binant and surface antigen recombinantsof type A influenza virus containing he-magglutinin of one parent and neuramini-dase of the other.The procedure involvesmixed infection of one inactivated parentand another infective parent virus in theallantoic sac of chicken embryos,neutro-lization and selection by proper antiseraand cloning by terminal dilution.One high yielding and many surfaceantigen recombinants have been obtainedby this simple and rapid procedure.Theapplication of surface antigen recom-binants to mono-specific antigenic analy-sis,to the assay of neuraminidase antibodyand to the antigenic characterization of1977 H1N1 virus is briefly described.