HIV/AIDS患者CCR5、CXCR4的表达与疾病进展的关系

Study on coreceptor expression and its correlation with disease progression in HIV/AIDS patients

目的 了解HIV AIDS患者淋巴细胞表面第二受体CCR5、CXCR4的表达 ,分析其与疾病进展的关系 ,探讨HIV感染的免疫基础。方法 收集 33例HIV AIDS患者及 13例健康对照的抗凝全血 ,用流式细胞仪检测第二受体CCR5、CXCR4的表达 ,并分析第二受体表达与病毒载量、CD4 + T淋巴细胞绝对值及T淋巴细胞活化 (HLA DR+ CD38+ )的相关性。结果 艾滋病组CD4 + 、CD8+ T淋巴细胞表面CCR5表达高于无症状HIV 1感染组及健康对照 (P <0 .0 0 1) ;艾滋病组CD8+ T淋巴细胞表面CXCR4表达低于健康对照 (P <0 .0 1)。HIV AIDS患者CD4 + 、CD8+ T淋巴细胞表面CCR5的表达与病毒载量明显正相关 (P <0 .0 1) ;与CD4 + T淋巴细胞绝对值明显负相关 (P <0 .0 1) ,与T淋巴细胞活化(HLA DR+ CD38+ )水平明显正相关 (P <0 .0 0 1)。结论 HIV 1感染者第二受体CCR5的表达与机体对HIV的免疫反应及疾病进展密切相关。

Objective To investigate the coreceptor CCR5, CXCR4 expression on T lymphocytes and to study their association with disease progression. Methods Thirty three HIV-1 infected patients and 13 normal controls were selected and flow-cytometry was used to detect the coreceptor CCR5, CXCR4 expressions in whole blood samples taken from the patients and the controls. Results The CCR5 expression on CD4 +, CD8 + T lymphocytes of AIDS group was higher than that of asymptomatic HIV-1 infected patients and normal controls ( P < 0.001 ); The CXCR4 expression on CD8 + T lymphocytes of AIDS group was lower than that of normal controls ( P <0.001); The CCR5 expression on CD4 +, CD8 + T lymphocytes of HIV-1 infected patients significantly correlated with viral load ( P <0.01), CD4 + T cell absolute count ( P <0.01) and activation of CD4 +, CD8 + T cells (HLA-DR + CD38 +) ( P <0.001). Conclusion The expression of CCR5 is significantly correlated with the immune system reaction toward HIV and disease progression in HIV-1 infected patients.