摘要
A new quantitative determination method of proteins using beryllon ?by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon ?can bind with human serum albumin (HSA) to form an electro-inactive supermolecular complex. Beryllon ?has a well-defined voltammetric reduction peak at 0.38 V (vs. SCE) and the addition of protein in this solution can cause the decrease of the reductive peak current. Based on the decrease of the reduction peak current, a new electrochemical method for the determination of HSA was established with linear range of 1.0~40.0 mg/L and the detection limit of 1.0 mg/L. This method is further applied to the determination of real sample of healthy human serum.
A new quantitative determination method of proteins using beryllon ?by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon ?can bind with human serum albumin (HSA) to form an electro-inactive supermolecular complex. Beryllon ?has a well-defined voltammetric reduction peak at 0.38 V (vs. SCE) and the addition of protein in this solution can cause the decrease of the reductive peak current. Based on the decrease of the reduction peak current, a new electrochemical method for the determination of HSA was established with linear range of 1.0~40.0 mg/L and the detection limit of 1.0 mg/L. This method is further applied to the determination of real sample of healthy human serum.
基金
supported by the National Natural Science Foundation of China(Grant No.20075013)
the Younger Foundation of Qingdao University of Science and Technology(Grant No.22000219).
