肥大细胞膜稳定剂对实验性重症急性胰腺炎的治疗作用研究

The therapeutic effect of mast cell membrane stabilizer on severe acute pancreatitis

目的 探讨肥大细胞 (m ast cell,MC)在重症急性胰腺炎 (SAP)时的致病作用 ,了解肥大细胞膜稳定剂对 SAP的治疗作用。方法 将 15只健康雄性 SD大鼠随机分为 3组 :假手术组、SAP组及 MC膜稳定剂处理组。 MC膜稳定剂处理组应用 MC膜稳定剂色甘酸钠 ,以 5 0 m g/ kg的用量于制模前 30 min注入大鼠腹腔进行预处理。使用胆管注射 5 %牛磺胆酸钠法诱发 SAP,观察各组大鼠血清淀粉酶、胰腺及肺组织病理变化、组织髓过氧化物酶 (MPO)活性以及肺干湿重比的变化情况。结果 SAP组的淀粉酶水平为 2 5 0 0 .0 ± 2 2 7.7U/ L,胰腺髓过氧化物酶活性为 0 .4 1± 0 .0 1U/ g湿重 ,肺干湿重比为4 .5 8± 0 .2 9,胰腺病变评分为 6 .4 ± 0 .5 ,均较假手术组明显增高 (P<0 .0 5 )。而使用 MC膜稳定剂预处理后造模 ,其淀粉酶水平降为 1894 .4 0 ± 12 4 .37U/ L,胰腺髓过氧化物酶活性为 0 .38± 0 .15 U/ g湿重 ,肺干湿重比为 4 .35 ± 0 .13,胰腺病变评分为 3.4 ± 0 .5 ,均较 SAP组明显减轻 (P<0 .0 5 )。结论 肥大细胞可能在 SAP发病过程中起着重要的作用 ,MC膜稳定剂对 SAP可能有治疗作用。

Objective To investigate the pathogenic role of mast cell(MC) in the course of severe acute pancreatitis(SAP) and the therapeutic effect of mast cell membrane stabilizer on SAP. Methods After pretreatment of rats with MC membrane stabilizer,sodium cromoglycate (SCG) at the dose of 50 mg/kg,SAP was induced by injection of 5% sodium taurocholate into the biliopancreatic duct. The severity of SAP was assessed by serum amylase level,pancreatic myeloperoxidase (MPO) level and the dry/wet weight ratio of lung. The degree of pancreatic impairment was also evaluated histologically. Results The levels of serum amylase (2 500.0 ± 227.7 U/L) and MPO (0.41 ± 0.01 U/g WW) and the dry/wet weight ratio of lung (4.58 ± 0.29) was increased significantly during the course of SAP without pretreatment of SCG. Administration of SCG significantly reduced the levels of serum amylase(1 894.40 ± 124.37 U/L) and MPO (0.38 ± 0.15 U/g WW) and the dry/wet weighty ratio of lung(4.35 ± 0.13),compared with those of SAP group ( P < 0.05). Conclusions Mast cells may play a crucial role in the development of sodium taurocholate-induced SAP,and SCG may have beneficial effects on SAP by stabilizing MC.