复方西红花膏对损伤组织bFGF mRNA表达的影响

The Effect of Basic Fibroblast Growth Factor mRNA (bFGF mRNA) Expressionby FFXHHG in the Wounded Tissue

目的 :研究复方西红花膏对损伤组织 b FGF m RNA基因表达的影响 ,探讨其促进损伤组织修复的作用机理。方法 :采用打击造模的方法 ,将 60只 Wistar大白鼠造成急性软组织损伤模型。随机将造模后动物分为空白对照组 ;阳性药物 (万花油 )对照组 ;复方西红花膏治疗组。观察用药 2天、4天、7天、1 0天 b FGF m R-NA水平的改变。结果 :复方西红花膏早期能提高损伤组织中 b FGF m RNA基因表达水平 ,且复方西红花膏组b FGF m RNA基因表达水平下降比其它两组缓慢。结论 :复方西红花膏通过调节 b FGF m RNA水平 ,直接刺激成纤维细胞及细胞外基质的蛋白合成 ,形成胶原纤维。并与相应的受体结合 ,诱导内皮细胞的迁移 ,促进毛细血管的增生 。

Objective:To study the effect of FFXHHG to bFGF mRNA expression in wounded tissue in order to elucidate the mechanism of FFXHHG promote the rehabilitation of the wounded tissue.Methods:According to the method of building models by beating, sixty wistar mouse got acute soft tissue's lesion. Then they were divided randomly into three groups: group A, used nothing; group B, healed with positive pharmaceutical; group C, coped with FFXHHG. Both group A and group B were control groups, while group C was experimental group. The level of bFGF mRNA expression was observed respectively at 2, 4, 7 and 10 days since the mouse were dealt with pharmaceutical.Results: FFXHHG could enhance the levels of bFGF mRNA expression in wounded tissue, while the level in FFXHHG decreased more slowly than the other two.Conclusion:The protein synthesis in fibroblast and extracellular matrix was stimulated directly by FFXHHG to regulate bFGF mRNA expression,and formed collagen fibril later. FFXHHG could be combined with the relevant receptors, induce endotheliocyte migration, improve the capillary hyperplasia and formed fibrocyte.