快速筛选苏云金杆菌高毒力菌株的有效方法

A Rapid and Efficient Method to Screen Highly Toxic Bacillus thuringiensis Strains

对7株苏云金芽孢杆菌菌株进行了cry1基因检测、晶体形态观察、晶体蛋白SDS PAGE以及杀虫活性检测。结果表明,综合菌株的PCR结果、晶体形态和晶体蛋白图谱结果可以预测其杀虫特性。根据菌株预测的杀虫特性进行有目的的生物测定,可以快速、准确地筛选到对特异害虫具高毒力的菌株。

Seven Bacillus thuringiensis strains were studied upon their cry1 gene types, crystal shapes, crystal protein patterns and insecticidal activities. Hereby a rapid and efficient method to screen highly toxic strains is summarized. That is to predict the insecticidal activities through holistic analysis of crystal shapes, cry gene types and protein patterns, afterwards to effectively screen highly toxic strains by directional bioassays.