五种植物源提取物对博物馆展厅空气来源细菌的抑制效果

Inhibition of five plant-derived extracts on airborne bacteria sampled in a museum exhibition hall

文物保存及展览环境中的细菌对于文物劣化的作用日益凸显。因此,开展空气微生物的净化工作对于文物安全具有重要意义。为此,本研究利用5种植物源提取物(Plant-derived extracts)作为净化剂,对博物馆展厅内采集到的空气微生物进行平板表面的气相熏蒸,进而通过活菌计数法和基于细菌16Sr RNA基因的聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术,评价植物源提取物在不同使用剂量下对空气细菌的抑制效果。结果显示,抑菌效果最好的植物源提取物为柠檬醛,最弱的为香叶油。经高剂量组柠檬醛、香茅醇和芳樟醇熏蒸,空气细菌总菌落数可分别下降98.53%,98.53%,97.95%,同时空气中细菌的丰富度和多样性指数出现显著下降。此外,PCR-DGGE结果提示,柠檬醛和香茅醇复配可进一步提高抑菌效果。植物源提取物作为博物馆展厅内的空气微生物净化剂具有较好的应用前景。

In addition to usual concerns about mold-induced deterioration, the potential impacts of bacteria in cultural relic preservation and exhibition environments have received more and more attention. Removal or reduction of airborne microorganisms inside museum exhibition halls would be helpful to preserve cultural relics. In our study, five plant-derived extracts were used as purifying agents to fumigate, in the plate, airborne microorganisms sampled from a museum exhibition hall. In order to evaluate the effects of inhibition on airborne microorganisms, the bacteria were quantified by pure culture method, and the bacterial compositions were characterized by the polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE) technique. The results show that citral had the best bacteriostatic effect and the weakest one was geranium oil. After fumigation with high doses of citral, citronellol and linalool, the total bacterial colonies were found decline by 98.53%, 98.53% and 97.95%, respectively. As a result, the richness and diversity index of airborne bacteria also declined significantly. In addition, the PCR-DGGE profile indicates that citral and citronellol might further improve the antibacterial effect. Our study has demonstrated that, as a kind of purifying agent for airborne microorganisms, plant-derived extracts have good prospects for decontamination of museum exhibition halls.