大豆作图群体检验与调整后构建的遗传图谱

A Soybean Genetic Linkage Map Constructed After the Mapping Population Being Tested and Adjusted

应用RFLP、SSR、AFLP对大豆重组近交系群体NJRIKY的 2 0 1个家系进行分析。根据RFLP标记的分析结果 ,通过模拟群体抽样标准法 (SPSC)对试验群体进行了检验与调整。结果表明 ,试验群体偏分离的家系较多 ,且整体偏向于亲本科丰 1号。汰除偏分离家系后 ,群体减少为 184个家系。对 3种分子标记进行连锁分析 ,结果表明 ,RFLP和SSR标记在基因组中的位置相对稳定 ,可以作为锚定标记 ,有利于连锁群的归并和不同图谱的比较整合 ;而AFLP标记容易出现聚集现象 ,从而造成连锁群上出现很大的空隙。以RFLP、SSR和形态等标记构建了包含2 2个连锁群的图谱 ,共 2 5 6个标记 ,总遗传距离为 30 5 0 .9cM。与未调整群体作图结果相比 ,19个标记及 2个连锁群有根本性改变。该图谱为基因定位。

RFLP, SSR and AFLP markers were used to analyze the 201 families of the soybean RIL population NJRIKY. By using the Simulated Population Sampling Criterion method (SPSC), the population was found to lean to the parent Kefeng1. After deleting the biased families, the population size was reduced from 201 to 184 families. In the construction of the genetic linkage map, RFLP and SSR markers showed more stable than AFLP markers and could be used as anchor markers. After adjustment of the mapping population, a genetic map with 256 RFLP, SSR and morphological markers, consisting 22 linkage groups, covering 3 050.9 cM was established. In comparison with the unadjusted one, the adjusted map showed substantial change on two linkage groups and 19 markers. The adjusted map could be used in mapping genes.