氰戊菊酯抗性和敏感品系棉蚜部分钠离子通道基因的克隆

Cloning of Partial Sodium Channel Gene From Strains of Fenvalerate-resistant and Susceptible Cotton Aphid(Aphis gossypii Glover)

在室内选育出了对氰戊菊酯抗性达 199.5 4倍的棉蚜品系。设计了 3条简并引物进行嵌套式PCR扩增 ,得到了氰戊菊酯敏感品系和抗性品系棉蚜钠离子通道基因IIS4 IIS6区的基因片段。序列分析表明 ,氰戊菊酯抗性品系棉蚜没有发生kdr或super kdr突变 ,但是与敏感品系相比 ,抗性品系钠离子通道基因IIS4 IIS6区有 2个核苷酸发生了突变 (T2A和A34T) ,并由此导致了 1个氨基酸的突变 (M12L) 。

The strain of fenvalerate-resistant cotton aphid was selected using fenvalerate insecticide in the laboratory. The resistance factor of the strain was 199.54. Three degenerate primers were designed and used to perform PCR amplification. A cDNA encoding partial sodium ion channel gene was cloned from the fenvalerate-susceptible and -resistant strains. There were two nucleotide acid differences between fenvalerate-resistant strain and -susceptible strain, resulting in an amino acid mutation (Met→Leu). It is predicted that the mutation is related to the fenvalerate resistance of cotton aphid.