摘要
基因表达系列分析技术 (SAGE)是一种以测序为基础 ,采用数字化分析手段 ,在转录物水平上研究细胞或组织基因表达模式的有效工具。该技术不仅能够全面地分析特定组织或细胞表达的基因 ,获得这些基因表达丰度的数量信息 ,还可比较不同组织、不同时空条件下基因表达的差异 ,从而发现新基因。SAGE技术在植物方面的应用相对较少 ,但进展很快。本文着重介绍了SAGE在植物基因表达分析中的应用 ,分析SAGE所存在的问题。
Serial analysis of gene expression(SAGE)is a powerful sequence-based tool, which allows the analysis of gene expression patterns in cell or tissue at the level of transcripts with digital analysis. It can be used not only to over ally analyze the genes that expressed in specific cells or tissues, in order to get the quantity information of the expressed genes, but also to simultaneously compare the differential genes expressed in different tissues and conditions for gene mining. The application of SAGE is fairly little in plant, but increases rapidly. In the future SAGE will be more and more used in plant functional genomies. In this review, the application and improvement of SAGE in plant genomic research are been introduced.
出处
《中国农业科学》
CAS
CSCD
北大核心
2003年第11期1233-1240,共8页
Scientia Agricultura Sinica
