中国芸芥遗传多样性RAPD标记分析

Genetic Diversity Analysis of Yunjie (Eruca sativa Mill.) in China by RAPD Marker

选择我国不同生态地区有代表性的芸芥材料 ,利用RAPD标记技术进行分子标记差异分析 ,根据遗传相似系数计算不同材料RAPD数据的遗传距离矩阵 ,按UPGMA方法对研究材料进行了聚类。研究结果表明 ,我国芸芥具有比较大的遗传多样性 ,12个引物共扩增出 131条DNA带 ,其中 10 5条表现出多态性 ,占总带数的 80 .15 % ,不同生态类型地区来源的材料有各自的特征带。 2 0个材料的遗传距离在 0 .1178～ 0 .4 994之间 ,其中以和田芸芥与其它材料的遗传距离最大 ,而神池芸芥与朔州芸芥遗传距离最小。聚类分析表明 ,2 0个材料可分为 4组 ,其中和田芸芥和四川芸芥各自列为一组 ,和田芸芥可能是我国芸芥的一种新类型。

Twenty Yunjie (E.sativa Mill.) ecotypes samples selected from different regions of China were studied on their genetic diversity with 12 random decamer primers filtered from 64 decamer primers by RAPD markers (random amplified polymorphic DNA procedures). The results indicated that Chinese Yunjie showed abundant genetic diversity. A total of 131 DNA bands were observed and 105 of them were polymorphic bands. An average proportion of polymorphic loci was 80.15%. Most of Yunjie come from different ecotopes showed their special DNA bands. Cluster analysis by unweighted pair group method of arithmetic means (UPGMA) suggested that 20 ecotypes could be divided into 4 groups with genetic distance from 0.1178-0.4994. Genetic distance between Shuozhou Yunjie and Shenchi was 0.1178, and that between Hetian Yunjie and Xiliang was 0.4994. Hetian Yunjie may be a new type of Yunjie in China. The groups have relation to the geographic origin and the morphologic character of Yunjie.