徐医附院2014年血培养病原菌分布和耐药性

Blood culture pathogens distribution and drug resistance from affiliated hospital of Xuzhou medical college in 2014

目的:监测徐州医学院附属医院2014年血培养阳性病原菌的耐药性,指导临床合理用药。方法:收集徐州医学院附属医院2014年348份血培养阳性病原菌;细菌鉴定采用Application Programming Interface(API)系统,细菌药物敏感性测定采用纸片扩散法,WHONET5.6软件进行数据分析。结果:血培养阳性标本中,革兰阳性球菌占48.9%,主要为金黄色葡萄球菌(50.0%),革兰阴性杆菌占40.0%,主要为大肠埃希菌(51.8%)。血培养送检科室多来自于重症监护病房(25.6%)。Methicillin Resistant Staphylococcus Aureus(MRSA)的检出率为54.8%。未发现耐万古霉素的菌株。对大肠埃希菌敏感率较高的有亚胺培南和美洛培南,都为100.0%。结论:我院血流感染病原菌种类复杂,建立健全细菌耐药性监测体制对了解细菌耐药性变迁,指导临床合理用药有重要意义。

Objective: To monitor the drug resistance of blood culture positive pathogenic bacteria from Affiliated Hospital of Xuzhou Medical College in 2014 and to guide clinical medication more reasonable. Methods: A total of 348 samples of blood culture positive pathogenic bacteria were collected from Affiliated Hospital of Xuzhou Medical College in 2014. Bacterial identification was performed by Application Programming Interface(API) system. Drug sensitivity was detected by disc diffusion method. WHONET5.6 software was used for data analysis. Results:The blood culture positive specimens, Gram-positive cocci was 48.9%, mainly for Staphylococcus aureus(50.0%), and Gram negative bacilli was 40.0%, mainly for Escherichia coli(51.8%). Blood culture inspection department mainly from the intensive care unit was 25.6%. The detection rate of Methicillin Resistant Staphylococcus Aureus(MRSA) was 54.8%. No vancomycin resistant strain was found. With high sensitivity to Escherichia coli are imipenem and meropenem, and it is 100.0%. Conclusion: The hospital’s bloodstream infection pathogenic bacteria species is complex, and establishing and perfecting the bacterial drug resistance surveillance system has important signii cance to understand of bacterial resistance to change, guide clinical rational and standardized medication.