昆明小鼠原核胚在不同培养液中的体外发育

In vitro Developmental Capacity of Kunming Mouse Pronuclear Embryos in Different Culture Medium

目的 优化昆明小鼠原核胚胎体外培养系统 ,提高胚胎发育率。方法 小鼠经超排获得原核期胚胎 ,制备小鼠输卵管上皮共培养系统 ,使用M16、CZB和KSOM培养液进行体外培养 ,并对体内和体外发育的囊胚细胞计数。结果 在KSOM和CZB中添加胎牛血清能显著提高胚胎囊胚发育率 (14 71%对 85 71% ;6 4 5 %对10 81% ) ;输卵管上皮共培养可以提高胚胎的卵裂率和囊胚发育率 ,同时提高胚胎质量和同步发育 ,小鼠胚胎在KSOMFBS中囊胚发育率达 85 19% ,显著高于CZB和M16。结论 在小鼠输卵管上皮共培养条件下 ,KSOMFBS能够很好支持昆明小鼠原核期胚胎体外发育。

Objective To establish a kind of culture system to improve %in vitro% development of Kunming mouse pronuclear embryos. Methods Mouse pronuclear embryos were obtained through superovulation, then the normal morphologic zygotes were selected and cultured in M16, CZB and KSOM respectively, or co_cultured with mice oviduct epithelia. The cell number of blastocysts obtained from in vito/in vitro were counted. Results The ratio of blastocyst formation was improved in KSOM and CZB by addition of fetal bovine serum(14.71% vs 85.71%,6.45% vs 10.81%). Co_culture system increased percentage of cleavage and blastocyst formation, and was advantageous for embryos quality and synchronized development. Conclusion Mouse pronuclear embryos developed in vitro highly in KSOM-{FBS} cocultured with oviduct epithelia.