NJS小鼠与其亲本小鼠遗传多样性的RAPD分析

RAPD analysis of Genetic Construction of NJS and Its Relative Mice

目的 分析NJS及其亲本小鼠的遗传结构。方法 筛选出 5条随机引物对NJS及其亲本小鼠的遗传结构进行RAPD分析 ,并对NJS小鼠个体之间的基因组DNA进行相似性分析。结果 两种小鼠均有相同的扩增片段且产生了各自的特异性条带 ;NJS小鼠不同个体间拥有的RAPD条带也具有差异 ,但拥有相同条带的个体小鼠比率较高。该群体小鼠的相似系数 (F)为 0 92 7(0 880～ 0 980 )。结论 RAPD分析获得的多态性可用于NJS与其亲本小鼠之间的遗传分析 ;而且NJS小鼠个体间具有较好的遗传一致性和遗传稳定性 ,其群体分化程度处在一个较低的水平。

Objective To analyze the genetic type of NJS and its relative mice.Methods Five 10bp random primers were used to amplify DNA of NJS and its relative mice and DNA analyzed among individuals in the population of NJS.Results There were several fragments of genetic DNA shared by two mice but specific fragments were seen.Different individual NJS had various bands,but the frequency of common bands was high.Based on RAPD patterns,similarity index(F) was 0.927(0.880-0.980).Conclusion RAPD could be used to distinguish NJS and its relative mice. There is higher degree of homogeneity and stability in heredity among individuals in the population of NJS and the degree of population differentiation is at a lower level.