抗纤Ⅰ号等方剂和硒抗大鼠肝纤维化作用的实验研究

Experimental study of anti-fibrosis prescription herb drugs and selenium on hepatic fibrosis in rat

目的 :研究抗纤Ⅰ号、抗纤Ⅱ号、抗纤Ⅲ号及各方剂加硒对大鼠肝纤维化治疗作用和免疫调节作用 ,并从分子水平对其机制进行探讨。方法 :利用四氯化碳、胆固醇等复合因素制造大鼠肝纤维化模型 ,设立空白对照组、肝纤维化模型组、模型自然恢复组、西药组及中药治疗组 ,中药灌胃治疗 4 2d ,检测病理学指标、血清酶学指标、血清学肝纤维化指标、免疫学指标 ,以及肝组织TGF β1mRNA表达量的变化。结果 :各项检查均显示抗纤Ⅰ号具有很好的抗肝纤维化的作用 ,其疗效优于抗纤Ⅱ号和抗纤Ⅲ号 ,抗纤Ⅱ号优于抗纤Ⅲ号 ,因此推测丹参为抗纤Ⅰ号的主药 ,鳖甲和郁金的辅助作用优于三棱和莪术。中药治疗组大鼠胸腺组织CD4、CD4/CD8均明显高于秋水仙碱组。加硒组与各方剂相比大鼠胸腺组织CD4、CD4/CD8均有升高 ,但差异无显著性。同时实验表明抗纤Ⅰ号能够明显抑制TGF β1mRNA的表达量。结论 :抗纤Ⅰ号对大鼠肝纤维化具有明确的治疗作用 ,且有提高机体免疫力的作用。其作用机制可能是通过下调TGF β1mRNA 。

OBJECTIVE To study the therapeutic effects of anti-fibrosis prescription herb drugs and selenium on hepatic fibrosis induced by carbon tetrachloride(CCl 4)in rats as well as to study the mechanisms of action on hepatic fibrosis of these herb drugs and selenium.METHODS CCl 4 was used to induce the hepatic fibrosis model of rat and anti-fibrosis prescription herb drugs and selenium were used to treat them. The serum indicators (ALT,AST,HA,LN) were determined and histopathological changes were observed by optical microscopy. CD 4 and CD 8 were examined by flow cytometry. The expressions of transforming growth factor(TGF-β 1) mRNA were detected by semi-quantified RT-PCR.RESULTS The serum indicators were significantly changed after the Chinese herb drugs were administrated into the rats with hepatic fibrosis. Saliva miltiorrhiza may be the main effective drug in anti-fibrosis herb drugs. The level of CD 4?CD 4/CD 8 in the Chinese herb drugs treatment group increased markedly after treatment of Chinese herb drugs. The expression of TGF-β 1 mRNA in the liver tissues of treatment group were markedly decreased.CONCLUSION The anti-fibrosis prescription herb drugs have a definite effect in treating hepatic fibrosis in rats by enhancing the level of immunity, inhibiting the TGF-β 1 mRNA expression.