柱切换高效液相色谱法测定人血浆中阿莫西林浓度

Determination of amoxicillin in human plasma by column-switching HPLC

目的 :建立柱切换高效液相色谱 (HPLC)法测定人血浆中阿莫西林浓度。方法 :血浆样品用乙腈沉淀蛋白及二氯甲烷反洗后 ,其水相进样于C18短柱 ,经 0 .0 2mol·L-1磷酸二氢钾 ( pH3.0 )在线净化 ,再由流动相 0 .0 2mol·L-1磷酸二氢钾 ( pH4 .6 ) 甲醇 ( 95∶5 )洗脱切入C8分析柱进行分离测定。结果 :线性范围 0 .2～ 2 0mg·L-1,净化回收率 81.7%～ 88.5 % ,方法回收率为 93.2 %～ 96 .7% ,日内RSD为 1.2 5 %～ 2 .5 7% ,日间RSD为 2 .74 %～ 9.4 9%。结论 :本法准确可靠 ,可用于阿莫西林体内血药浓度测定。

OBJECTIVE To develop a column-switching HPLC method for the determination of amoxicillin in human plasma.METHODS The plasma samples were precipitated by acetonitrile and the supernatant was vortexed with methylene chloride.The aqueous layer injected onto cleanup column was on-line washed with 0.02 mol·L -1 KH 2PO 4(pH 3.0 ),the residues were eluted by the mobile phase of 0.02 mol·L -1 KH 2PO 4 (pH 4.6 )-methanol (95∶5) onto analytical column for separation and determination.RESULTS The calibration linear range for amoxicillin in plasma was 0.2 ～20 mg·L -1. The recoveries of cleanup were 81.7% ～ 88.5% .The recoveries of methodology ranged from 93.2% ～ 96.7% .Inter-day RSD and intra-day RSD were 1.25% ～ 2.57 % and 2.74% ～ 9.49% respectively.CONCLUSION This method is accurate and reliable. It can be applied to the determination of amoxicillin in human plasma.