精氨酸血管加压素mRNA在不同免疫应答期大鼠下丘脑室旁核的表达变化

A Study of the Dynamic Relationship Between AVP mRNA Expression in PVN and Immuno-responsiveness in Rats

目的 研究大鼠下丘脑室旁核 (PVN)内精氨酸血管加压素 (AVP) m RNA的表达与免疫应答反应间的动态关系。方法 建立免疫增强及免疫抑制大鼠模型 ,应用原位杂交组织化学及图像分析技术检测免疫反应不同时期大鼠 PVN内 AVP m RNA的动态变化。结果 1免疫抑制组大鼠 PVN单个细胞内 AVP m RNA阳性杂交信号的平均面积明显低于免疫增强组及对照组 (P<0 .0 5 )。 2 PVN单个细胞内 AVP m RNA阳性杂交信号的平均灰度值在免疫反应高峰时显著低于免疫反应开始增强时 (P<0 .0 5 ) ,在免疫抑制最低时却明显高于免疫反应开始受抑制时 (P<0 .0 5 )。 3AVP m RNA阳性杂交信号在 PVN内所占的总面积在免疫反应高峰时显著大于免疫反应开始增强时和对照组 (P<0 .0 5 ) ,在免疫抑制最低时则明显小于免疫反应开始受抑制时 (P<0 .0 5 )。4 AVP m RNA阳性杂交信号在整个 PVN内的平均灰度值在免疫反应高峰时明显低于免疫反应开始增强时 (P<0 .0 5 ) ,在免疫抑制最低时却明显高于免疫反应开始受抑制时 (P<0 .0 5 )。结论 PVN内 AVP m RNA的含量与免疫应答反应的强度成正比 ,AVP基因的转录随免疫应答反应的变化而变化。

Objective To investigate the proper dynamic relationship between AVP mRNA expression in PVN and immune response. Methods Through the establishment of the immuno-enhancement and immuno-suppression rat models, the in situ hybridization histochemical methods and the images analytical techniques were used to detect the dynamic variations of AVP mRNA content of PVN during different periods of immune response. Results ① The average area of AVP mRNA positive signal in single cell of PVN in the immune suppression group was significantly smaller than that in the immune enhancement group and control group (P<0.05).② The average gray degree of AVP mRNA positive signal in single cell of PVN in the maximum period of immune response was lower than that in the incipient enhancement period of immune response (P<0.05). On the other hand, the average gray degree was clearly higher in the minimum period of immune response than that in the incipient suppression period of immune response (P<0.05). ③ The total area that the AVP mRNA-positive signal occupied in the whole PVN in the maximum enhancement period of immune response was significantly larger than that in the incipient enhancement period response and the control group (P<0.05).Conversely, it was evidently smaller in the minimum suppression period than in the incipient suppression period of immune response (P< 0.05 ).④ The average gray degree of AVP mRNA positive signal in the whole PVN in the maximum enhancement period of immune response was significantly lower than that in the incipient enhancement period and the control group (P<0.05). In turn, it was obviously higher in the minimum suppression period than in the incipient suppression period of immune response and the control group (P<0.05). Conclusion There is a positive relationship between the AVP mRNA content of PVN and the intensity of immune responsiveness, indicating that the situation of AVP gene transcription varies with the variation of immune responsiveness.