枣胚培养影响因素研究

Factors Influencing Young Embryo Culture of Chinese Jujube

以枣优良品种冬枣、金丝丰等为试材,对坐果后10～70d幼胚进行了培养,结果表明,胚龄是影响胚培养成功的关键性因素,暗处理对生根有利,低温处理(2～4℃,44d)能缩短成苗时间并提高成苗率,但成苗质量受到影响。20～40d幼胚成苗最适培养基为BA0.1mg/L+IBA0.5mg/L+NAA0.1mg/L+蔗糖50mg/L+LH500mg/L的MS培养基,成苗时间超过80d。55～70d幼胚在无激素培养基中亦能获得良好效果。无根苗转到添加BA0.1mg/L+IBA0.5mg/L+IAA0.1mg/L+蔗糖30g/L的MS培养基中可促进生根。生根苗转至无激素+蔗糖30g/L的MS培养基可获得健壮苗。

days old embryos of Ziziphus juj ub a cv.Dongzao and cv.Jinsifeng etc were used for studying the factors influenci ng embryo culture.The key factor influencing embryo culture was the age of embr yo.Dark treatment was beneficial to rooting.Low temperature treatment (2～ 4℃,44d)could shorten the time of raising plantlets and raise the successf ul rate but descended the quality of raised materials.The media of MS+BA0. 1mg/L+IBA0.5mg/L+NAA0.1mg/L+Sugar50g/L+LH500mg/L was s uitable for20～40days old embryo culture;55～70days old embryo could be cultured well in the media without plant growth regulator.The rootless cultu red materials should be transferred to MS+BA0.1mg/L+IBA0.5mg/L+I-A A0.1mg/L+Sugar30g/L to induce normal root system.For getting strong c ultured plantlets,the rooted ones should be fur-ther transferred to MS +Sugar of30g/L.