摘要
ISSR是一种基于微卫星序列发展起来的新的分子标记,具有简便、稳定、DNA多态性高等优点。ISSR标记呈孟德尔式遗传,大部分ISSR标记为显性标记。综合有关文献就ISSR的原理、操作及其在果树种质鉴定、遗传多样性检测、亲缘关系分析和遗传图谱构建等方面的应用和进展进行简要的阐述。
Inter-simple sequence repeat(ISSR)is a microsatellite-based technique being used for genetic and breeding studies as a new molecular marker.ISSR markers are a quick,simple and reliable method and may also generate greater DNA polymorphism.ISSR markers are inherited and segr egated in Mendelian fashion and most of which behave as dominant markers.This paper emphasizes on the principle and procedures of ISSR and its application to germplasm identification,ge-netic diversity detection,phylogenetic analysis, and genetic map construction in fruit trees.
出处
《果树学报》
CAS
CSCD
北大核心
2004年第1期54-58,共5页
Journal of Fruit Science
基金
国家自然科学基金资助项目(30270925)
关键词
ISSR
种质鉴定
遗传多样性检测
亲缘关系分析
遗传图谱构建
果树
Inter-simple sequence repeat(ISSR)
Germplasm identification
Genetic diversity detection
Phylogene tic analysis
Genetic map construction
Fruit trees
