摘要
以杜仲(EncommiaulmoidesOliver)当年生枝条的叶片、带芽茎段为外植体,基本培养基为MS,附加不同浓度的BA、KT和NAA.最佳芽诱导培养基为:MS+KT1 0mg/L+NAA0 1～0 5mg/L;MS+BA2 0mg/L+NAA0 1～0 5mg/L,最佳芽丛增殖培养基为:MS+KT2 0mg/L+NAA0 1～0 2mg/L;MS+BA1 0～2 0mg/L+NAA0 1～0 2mg/L;MS+KT2 0mg/L+IBA0 1～0 2mg/L;MS+BA1 0～2 0mg/L+IBA0 1～0 2mg/L,单芽增殖培养基:MS+KT1 0mg/L+NAA0 1mg/L,生根培养基:13MS+KT0 1mg/L+NAA0 5mg/L.
The leaves and annual shoot stems with a lateral buds of Encommia ulmoides Oliver were cultured on MS contained BA,KT and NAA at different levels.The optimal media of inducing buds is MS+KT?1.0?mg/L+NAA?0.1～0.5?mg/L;MS+BA?2.0?mg/L+NAA?0.1～0.5?mg/L.Best for proliferation of shoots is on MS+KT?2.0?mg/L +NAA?0.1～0.2?mg/L;MS+BA?1.0～2.0?mg/L+NAA?0.1～0.2?mg/L;MS+KT?2.0?mg/L+IBA?0.1～0.2?mg/L;MS+BA?1.0～2.0?mg/L+IBA?0.1～0.2?mg/L.Propagation for the cuttage in vitro is on MS + KT?1.0?mg/L+NAA?0.1?mg/L.Rootage is on 13?MS +KT?0.1?mg/L+NAA?0.5?mg/L.
出处
《西北师范大学学报（自然科学版）》
CAS
2004年第1期64-66,共3页
Journal of Northwest Normal University(Natural Science)
关键词
杜仲
离体培养
增殖
Encommia ulmoides Oliver
culture in vitro
proliferation
