血清学方法检测SARS病毒抗体的比较

A comparative study to detect the specific antibody of SARS patients by the serological methods

目的 :比较血清学方法 (间接ELISA法和双抗原夹心ELISA法 )对SARS患者特异性抗体的检出情况。方法 :采用间接法和双抗原夹心法分别检测健康人、发热非SARS患者、不同时期的SARS患者血清中的特异性抗体水平。结果 :双抗原夹心法的阳性检出率为 73.6 % ,而间接法的阳性检出率为 5 7.5 % ,前者对SARS患者各时期特异性抗体的阳性检出率高于间接法 ,特别是住院第 1周的阳性检出率为 30 % ,而间接法对住院第 1周的阳性检出率为 0 %。结论

Objective:To compare serological methods(the Indirect ELISA with the Double antigen sandwich ELISA) to detect the specific antibody in the serum of SARS patients. Methods:The levels of the specific antibody in the serum of SARS patients in varied period, the healthy and feverous patients without SARS were detected by the Indirect ELISA and the Double antigen sandwich method. Results:The maximal positive detection rate of the specific antibody in the serum of SARS patients is 73.6% by the Double antigen sandwich method, whereas the maximal positive detection rate of the specific antibody in the serum of SARS patients is 57.5% by the Indirect ELISA, and furthermore, the positive detection rates of the specific antibody in the serum of SARS patients in varied period by the Double antigen sandwich method are greater than that by the Indirect ELISA,especially the positive detection rates of the specific antibody in the serum of SARS patients with in first week of hospitalization by the Double antigen sandwich method is 30%, whereas, the positive detection rates of the specific antibody in the serum of SARS patients in first week of hospitalization by the Indirect ELISA is 0%.Conclusion: The Double antigen sandwich method is superior to the Indirect ELISA in detecting the specific antibody in the serum of SARS patients.